3D Mapping of OPT Gene Expression Patterns

We have developed a technique that allows 3D mapping of gene expression patterns captured using the 3D imaging technique Optical Projection Tomography (OPT).  A source OPT expression pattern (coloured components) is mapped onto a reference model (greyscale) and these can be viewed in any plane using the 3D viewer. 

The examples below show various genes in the Wnt signalling pathway.
Click on the thumbnails to show these expression patterns in the 3D viewer.

Gene Data Image Theiler Stage Stage Given 3D Expression ID Fzd1 TS17 10.5dpc 3D View EMAGE:6130 Fzd6 TS17 10.5dpc 3D View EMAGE:6121 Fzd7 TS17 10.5dpc 3D View EMAGE:6123 Fzd8 TS17 10.5dpc 3D View EMAGE:6125 Fzd9 TS17 10.5dpc 3D View EMAGE:6126 Fzd10 TS17 10.5dpc 3D View EMAGE:6128 Wnt1 TS17 10.5dpc 3D View EMAGE:6132 Wnt2 TS17 10.5dpc 3D View EMAGE:6134 Wnt3 TS17 10.5dpc 3D View EMAGE:6138 Wnt3A TS17 10.5dpc 3D View EMAGE:6141 Wnt4 TS17 10.5dpc 3D View EMAGE:6142 Wnt5A TS17 10.5dpc 3D View EMAGE:6144 Wnt6 TS17 10.5dpc 3D View EMAGE:6148 Wnt7A TS17 10.5dpc 3D View EMAGE:6150 Wnt8B TS17 10.5dpc 3D View EMAGE:6154 Wnt9B TS17 10.5dpc 3D View EMAGE:6157 Wnt16 TS17 10.5dpc 3D View EMAGE:6165

Movie showing various OPTs of Wnt genes (from Dr Paula Murphy) manually warped into the TS17 EMA53 reference model. This illustrates the powerful method by which warping into a common space allows users to discern overlaps and non-overlaps of differing genes.